Ontology highlight
ABSTRACT:
SUBMITTER: Hannanta-Anan P
PROVIDER: S-EPMC6311707 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Hannanta-Anan Pimkhuan P Chow Brian Y BY
ACS synthetic biology 20180604 6
As fast terminators of G-protein coupled receptor (GPCR) signaling, regulators of G-protein signaling (RGS) serve critical roles in fine-tuning second messenger levels and, consequently, cellular responses to external stimuli. Here, we report the creation of an optogenetic RGS2 (opto-RGS2) that suppresses agonist-evoked calcium oscillations by the inactivation of Gα<sub>q</sub> protein. In this system, cryptochrome-mediated heterodimerization of the catalytic RGS2-box with its N-terminal amphipa ...[more]