Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling.

ABSTRACT: Rho/Ras family small GTPases are known to regulate numerous cellular processes, including cytoskeletal reorganization, cell proliferation, and cell differentiation. These processes are also controlled by Ca²⁺, and consequently, cross talk between these signals is considered likely. However, systematic quantitative evaluation has not yet been reported. To fill this gap, we constructed optogenetic tools to control the activity of small GTPases (RhoA, Rac1, Cdc42, Ras, Rap, and Ral) using an improved light-inducible dimer system (iLID). We characterized these optogenetic tools with genetically encoded red fluorescence intensity-based small GTPase biosensors and confirmed these optogenetic tools' specificities. Using these optogenetic tools, we investigated calcium mobilization imme

SUBMITTER: Inaba H 

PROVIDER: S-EPMC7949103 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature