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Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking.


ABSTRACT: Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5' UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

SUBMITTER: Kim SC 

PROVIDER: S-EPMC6319552 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking.

Kim Sang Chul SC   Lee Eun-Hye EH   Yu Ji Hea JH   Kim Sang-Mi SM   Nam Bae-Geun BG   Chung Hee Yong HY   Kim Yeon-Soo YS   Cho Sung-Rae SR   Park Chang-Hwan CH  

Biomolecules & therapeutics 20190101 1


Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation <i>in vivo</i>. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein un  ...[more]

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