Ontology highlight
ABSTRACT:
SUBMITTER: Bolognin S
PROVIDER: S-EPMC6325628 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Bolognin Silvia S Fossépré Marie M Qing Xiaobing X Jarazo Javier J Ščančar Janez J Moreno Edinson Lucumi EL Nickels Sarah L SL Wasner Kobi K Ouzren Nassima N Walter Jonas J Grünewald Anne A Glaab Enrico E Salamanca Luis L Fleming Ronan M T RMT Antony Paul M A PMA Schwamborn Jens C JC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20181120 1
Parkinson's disease (PD)-specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient-specific neurons, derived from induced pluripotent stem cells carrying the LRRK2-G2019S mutation, is optimized in 3D microfluidics. The automated image analysis algorithms are implemented to enable pharmacophenomics in disease-relevant conditions. In contrast to 2D cultures, this 3D approach reveals robust endophenotypes. High-content imaging data sh ...[more]