Dataset Information

A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize.

ABSTRACT: Genomic deletions are pervasive in the maize (Zea mays L.) genome, and play important roles in phenotypic variation and adaptive evolution. However, little is known about the biological functions of these genomic deletions. Here, we report the biological function of a megabase-scale deletion, which we identified by position-based cloning of the multi-trait weakened (muw) mutant, which is inherited as a single recessive locus. MUW was mapped to a 5.16-Mb region on chromosome 2. The 5.16-Mb deletion in the muw mutant led to the loss of 48 genes and was responsible for a set of phenotypic abnormities, including wilting leaves, poor yield performance, reduced plant height, increased stomatal density, and rapid water loss. While muw appears to have resulted from double-stranded break repair that was not dependent on intragenomic DNA homology, extensive duplication of maize genes may have mitigated its effects and facilitated its survival.

SUBMITTER: Han X

PROVIDER: S-EPMC6325712 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize.

Han Xuesong X Qin Yao Y Yu Feng F Ren Xuemei X Zhang Zuxin Z Qiu Fazhan F

Genetics 20181102 1

Genomic deletions are pervasive in the maize (Zea mays L.) genome, and play important roles in phenotypic variation and adaptive evolution. However, little is known about the biological functions of these genomic deletions. Here, we report the biological function of a megabase-scale deletion, which we identified by position-based cloning of the multi-trait weakened (muw) mutant, which is inherited as a single recessive locus. MUW was mapped to a 5.16-Mb region on chro ...[more]

PMID: 30389804

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Project description:OBJECTIVE:Multiple sclerosis (MS) is a heterogenous, inflammatory disease of the central nervous system. Microbiota alterations in MS versus healthy controls (HC) are observed, but results are inconsistent. We studied diversity, enterotypes, and specific gut microbial taxa variation between MS and HC, and between MS subgroups. METHODS:Amplicon sequencing of the 16S ribosomal RNA V4 region (Illumina MiSeq) was used to evaluate alpha and beta diversity, enterotypes, and relative taxa abundances on stool samples. MS subgroups were based on phenotype, disease course modifiers, and treatment status. Results were controlled for recently identified confounders of microbiota composition. RESULTS:Ninety-eight MS patients and 120 HC were included. Microbial richness was lower in interferon-treated (RRMS_I, N = 24) and untreated relapsing-remitting MS during relapse (RRMS_R, N = 4) when compared to benign (BMS, N = 20; Z = -3.07, Pcorr = 0.032 and Z = -2.68, Pcorr = 0.055) and primary progressive MS (PPMS, N = 26; Z = -2.39, Pcorr = 0.062 and Z = -2.26, Pcorr = 0.071). HC (N = 120) and active untreated MS (RRMS_U, N = 24) showed intermediate microbial richness. Enterotypes were associated with clinical subgroups (N = 218, ?2 = 36.10, P = 0.002), with Bacteroides 2 enterotype being more prevalent in RRMS_I. Butyricicoccus abundance was lower in PPMS than in RRMS_U (Z = -3.00, Pcorr = 0.014) and BMS (Z = -2.56, Pcorr = 0.031), lower in RRMS_I than in BMS (Z = -2.50, Pcorr = 0.034) and RRMS_U (Z = -2.91, Pcorr = 0.013), and inversely correlated with self-reported physical symptoms (rho = -0.400, Pcorr = 0.001) and disease severity (rho = -0.223, P = 0.027). INTERPRETATION:These results emphasize the importance of phenotypic subcategorization in MS-microbiome research, possibly explaining previous result heterogeneity, while showing the potential for specific microbiome-based biomarkers for disease activity and severity.

Dataset Information

A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize.

Publications

A Megabase-Scale Deletion is Associated with Phenotypic Variation of Multiple Traits in Maize.

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