Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis.
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ABSTRACT:
SUBMITTER: Castel B
PROVIDER: S-EPMC6326418 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis.
Castel Baptiste B Tomlinson Laurence L Locci Federica F Yang Ying Y Jones Jonathan D G JDG
PloS one 20190109 1
Bacterial CRISPR systems have been widely adopted to create operator-specified site-specific nucleases. Such nuclease action commonly results in loss-of-function alleles, facilitating functional analysis of genes and gene families We conducted a systematic comparison of components and T-DNA architectures for CRISPR-mediated gene editing in Arabidopsis, testing multiple promoters, terminators, sgRNA backbones and Cas9 alleles. We identified a T-DNA architecture that usually results in stable (i.e ...[more]
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