Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3.
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SUBMITTER: Kuzhyk Y
PROVIDER: S-EPMC6328414 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
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Genome Engineering Approaches to Improve Nosokomycin A Production by <i>Streptomyces ghanaensis</i> B38.3.
Kuzhyk Yuriy Y Lopatniuk Maria M Luzhetskyy Andriy A Fedorenko Victor V Ostash Bohdan B
Indian journal of microbiology 20180925 1
Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by <i>Streptomyces ghanaensis</i> ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene <i>bldA</i> and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production. ...[more]
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