Unknown

Dataset Information

0

Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development.


ABSTRACT: In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-? and interleukin-1? was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.

SUBMITTER: Sun G 

PROVIDER: S-EPMC6331844 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development.

Sun Guijin G   Liu Kechun K   Wang Xue X   Liu Xiuhe X   He Qiuxia Q   Hsiao Chung-Der CD  

Molecules (Basel, Switzerland) 20151012 10


In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial p  ...[more]

Similar Datasets

| S-EPMC3000104 | biostudies-literature
| S-EPMC3196795 | biostudies-literature
| S-EPMC6789604 | biostudies-literature
| S-EPMC3596712 | biostudies-literature
| S-EPMC8113121 | biostudies-literature
| S-EPMC6309420 | biostudies-literature
| S-EPMC4446259 | biostudies-literature
| S-EPMC1220735 | biostudies-other
| S-EPMC1383735 | biostudies-literature
| S-EPMC6936149 | biostudies-literature