Dataset Information

Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.

ABSTRACT: Somatic embryogenesis (SE) and organogenesis have become leading biotechnologies for forest tree improvement and the implementation of multi-varietal forestry. Despite major advances in clonal propagation using these technologies, many forest tree species, such as conifers, show a low regeneration capacity. Developmental factors such as genotype, the type and age of the explant or tissue, and the age and maturity of the mother tree are limiting factors for the success of propagation programs. This review summarizes recent research on the molecular pathways involved in the regulation of key steps in SE and organogenesis of forest tree species, mainly conifers. The interaction between auxin and stress conditions, the induction of cell identity regulators and the role of cell wall remodeling are reviewed. This information is essential to develop tools and strategies to improve clonal propagation programs for forest tree species.

SUBMITTER: Diaz-Sala C

PROVIDER: S-EPMC6333695 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.

Díaz-Sala Carmen C

Frontiers in plant science 20190109

Somatic embryogenesis (SE) and organogenesis have become leading biotechnologies for forest tree improvement and the implementation of multi-varietal forestry. Despite major advances in clonal propagation using these technologies, many forest tree species, such as conifers, show a low regeneration capacity. Developmental factors such as genotype, the type and age of the explant or tissue, and the age and maturity of the mother tree are limiting factors for the success of propagation programs. Th ...[more]

PMID: 30687348

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Project description:The production of tree seedlings in forest nurseries and their use in the replanting of clear-cut forest sites is a common practice in the temperate and boreal forests of Europe. Although conifers dominate on replanted sites, in recent years, deciduous tree species have received more attention due to their often-higher resilience to abiotic and biotic stress factors. The aim of the present study was to assess the belowground fungal communities of bare-root cultivated seedlings of Alnusglutinosa,Betula pendula,Pinus sylvestris, Piceaabies and Quercusrobur in order to gain a better understanding of the associated fungi and oomycetes, and their potential effects on the seedling performance in forest nurseries and after outplanting. The study sites were at the seven largest bare-root forest nurseries in Lithuania. The sampling included the roots and adjacent soil of 2-3 year old healthy-looking seedlings. Following the isolation of the DNA from the individual root and soil samples, these were amplified using ITS rRNA as a marker, and subjected to high-throughput PacBio sequencing. The results showed the presence of 161,302 high-quality sequences, representing 2003 fungal and oomycete taxa. The most common fungi were Malasseziarestricta (6.7% of all of the high-quality sequences), Wilcoxinamikolae (5.0%), Pustularia sp. 3993_4 (4.6%), and Fusariumoxysporum (3.5%). The most common oomycetes were Pythiumultimum var. ultimum (0.6%), Pythiumheterothallicum (0.3%), Pythium spiculum (0.3%), and Pythiumsylvaticum (0.2%). The coniferous tree species (P.abies and P. sylvestris) generally showed a higher richness of fungal taxa and a rather distinct fungal community composition compared to the deciduous tree species (A.glutinosa, B. pendula, and Q.robur). The results demonstrated that the seedling roots and the rhizosphere soil in forest nurseries support a high richness of fungal taxa. The seedling roots were primarily inhabited by saprotrophic and mycorrhizal fungi, while fungal pathogens and oomycetes were less abundant, showing that the cultivation practices used in forest nurseries secured both the production of high-quality planting stock and disease control.

Dataset Information

Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.

Publications

Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.

Similar Datasets

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