Multiplexed Lipid Bilayers on Silica Microspheres for Analytical Screening Applications.

ABSTRACT: Most druggable targets are membrane components, including membrane proteins and soluble proteins that interact with ligands or receptors embedded in membranes. Current target-based screening and intermolecular interaction assays generally do not include the lipid membrane environment in presenting these targets, possibly altering their native structure and leading to misleading or incorrect results. To address this issue, an ideal assay involving membrane components would (1) mimic the natural membrane environment, (2) be amenable to high-throughput implementation, and (3) be easily multiplexed. In a step toward developing such an ideal target-based analytical assay for membrane components, we present fluorescently indexed multiplexed biomimetic membrane assays amenable to high-throughput

SUBMITTER: Fernandez Oropeza N 

PROVIDER: S-EPMC6342469 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature