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Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

ABSTRACT: Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

SUBMITTER: Strauss S

PROVIDER: S-EPMC6345375 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

Strauss Sebastian S Nickels Philipp C PC Strauss Maximilian T MT Jimenez Sabinina Vilma V Ellenberg Jan J Carter Jeffrey D JD Gupta Shashi S Janjic Nebojsa N Jungmann Ralf R

Nature methods 20180820 9

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capabil ...[more]

PMID: 30127504

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