Ontology highlight
ABSTRACT:
SUBMITTER: Jungmann R
PROVIDER: S-EPMC4153392 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Jungmann Ralf R Avendaño Maier S MS Woehrstein Johannes B JB Dai Mingjie M Shih William M WM Yin Peng P
Nature methods 20140202 3
Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures. ...[more]