Dataset Information

High Expression of long non-coding RNA PVT1 predicts metastasis in Han and Uygur Patients with Gastric Cancer in Xinjiang, China.

ABSTRACT: To analyze the level and diagnostic value of plasmacytoma variant translocation 1 (PVT1) in gastric cancer (GC) of Han and Uygur in Xinjiang, China, we collected 42 GC and 47 normal gastric tissues and performed tissue microarray. In situ hybridization was used to detect PVT1, while immunohistochemistry was used to analyze c-myc. The relationship between PVT1, c-myc and clinical pathological features was investigated. We then analyzed the expression of PVT1 in six GC cell lines. RNA interference was used to silence PVT1 in BGC823 and AGS cells. c-myc was detected by western blotting after silencing PVT1, while proliferation, invasion and migration ability were also analyzed. We found that PVT1 and c-myc were highly expressed in both Han and Uygur GC tissues. In Han GC, PVT1 was correlated with lymph node metastasis and primary tumor site. In Uygur GC, both PVT1 and c-myc were correlated with lymph node metastasis and clinical staging. PVT1 was positively correlated with c-myc. BGC823 and AGS cells exhibited high levels of PVT1. When PVT1 expression was silenced, the expression of c-myc decreased, while migration and invasion ability were also decreased in cells. PVT1 could therefore be a potential biomarker to predict the metastatic tendency of GC in both Han and Uygur patients.

SUBMITTER: Ren X

PROVIDER: S-EPMC6345741 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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High Expression of long non-coding RNA PVT1 predicts metastasis in Han and Uygur Patients with Gastric Cancer in Xinjiang, China.

Ren Xianxian X Cao Dongdong D Yang Li L Li Xia X Zhang Wei W Xiao Yongbiao Y Xi Yu Y Li Feng F Li Dongmei D Pan Zemin Z

Scientific reports 20190124 1

To analyze the level and diagnostic value of plasmacytoma variant translocation 1 (PVT1) in gastric cancer (GC) of Han and Uygur in Xinjiang, China, we collected 42 GC and 47 normal gastric tissues and performed tissue microarray. In situ hybridization was used to detect PVT1, while immunohistochemistry was used to analyze c-myc. The relationship between PVT1, c-myc and clinical pathological features was investigated. We then analyzed the expression of PVT1 in six GC cell lines. RNA interference ...[more]

PMID: 30679629

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Project description:ObjectivesTo estimate the prevalence, ethnic differences and associated risk factors of myopic maculopathy in Han and Uygur adults in Xinjiang, China.Design and settingA cross-sectional study with multistage, stratified cluster sampling method was conducted in Xinjiang, China.ParticipantsA total of 4023 Han and Uygur participants aged 40 years and older were eligible for the study.Outcome measuresThe association between myopic maculopathy and its risk factors was screened using the generalised estimating equation (GEE) model. We also investigated whether ethnic differences exist between Han and Uygur populations affected by myopic maculopathy. Myopic maculopathy was defined in accordance with International Photographic Classification and Grading System for Myopic Maculopathy.ResultsA total of 3044 subjects (5946 eyes) were included in our study (1736 Han and 1308 Uygur individuals). The participants consisted of 1256 (41.3%) men and 1788 (58.7%) women. The average age was 52.2±9.4 years, and the mean spherical equivalent (SE) was -0.18±2.31 dioptre (D). Myopic maculopathy was detected in 198 eyes of 138 participants. The age-adjusted prevalence of myopic maculopathy reached 5.8% (95% CI 4.8 to 6.8). In the GEE model, myopic maculopathy was significantly associated with old age (per year; OR: 1.16; 95% CI 1.13 to 1.19; p<0.001) and myopic SE (per -1 D; OR: 1.48; 95% CI 1.40 to 1.56; p<0.001). No ethnic differences were detected between Han (98, 5.7%) and Uygur population (40, 3.1%) in terms of the prevalence of myopic maculopathy (OR=0.89; 95% CI 0.53 to 1.48; p=0.64). Neither urbanisation (p=0.38) nor the level of education (p=0.92) was associated with myopic maculopathy.ConclusionsA high age-adjusted prevalence of myopic maculopathy was observed in Han and Uygur populations in Xinjiang, China. Old age and high degree of myopic refraction were independent risk factors for myopic maculopathy. No ethnic differences were detected in Han and Uygur populations affected by myopic maculopathy.

Dataset Information

High Expression of long non-coding RNA PVT1 predicts metastasis in Han and Uygur Patients with Gastric Cancer in Xinjiang, China.

Publications

High Expression of long non-coding RNA PVT1 predicts metastasis in Han and Uygur Patients with Gastric Cancer in Xinjiang, China.

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