High-Throughput Assessment of Structural Continuity in Biologics.
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ABSTRACT:
SUBMITTER: Musetti C
PROVIDER: S-EPMC6349355 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
High-Throughput Assessment of Structural Continuity in Biologics.
Musetti Caterina C Bean Mark F MF Quinque Geoffrey T GT Kwiatkowski Christopher C Szewczuk Lawrence M LM Baldoni John J Zajac Matthew A MA
Analytical chemistry 20180208 4
We demonstrate a high-throughput chemoprinting platform that confirms the consistency in the higher-order structure of protein biologics and is sensitive enough to detect single-point mutations. This method addresses the quality and consistency of the tertiary and quaternary structure of biologic drug products, which is arguably the most important, yet rarely examined, parameter. The method described uses specific small-molecule ligands as molecular probes to assess protein structure. Each libra ...[more]
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