Ontology highlight
ABSTRACT:
SUBMITTER: Schmidlin T
PROVIDER: S-EPMC6838672 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Schmidlin Thierry T Debets Donna O DO van Gelder Charlotte A G H CAGH Stecker Kelly E KE Rontogianni Stamatia S van den Eshof Bart L BL Kemper Kristel K Lips Esther H EH van den Biggelaar Maartje M Peeper Daniel S DS Heck Albert J R AJR Altelaar Maarten M
Cell systems 20190911 4
Aberrant kinase activity has been linked to a variety of disorders; however, methods to probe kinase activation states in cells have been lacking. Until now, kinase activity has mainly been deduced from either protein expression or substrate phosphorylation levels. Here, we describe a strategy to directly infer kinase activation through targeted quantification of T-loop phosphorylation, which serves as a critical activation switch in a majority of protein kinases. Combining selective phosphopept ...[more]