Ontology highlight
ABSTRACT:
SUBMITTER: Rousset F
PROVIDER: S-EPMC6350225 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Rousset Francis F Salmon Patrick P Bredl Simon S Cherpin Ophélie O Coelho Marta M Myburgh Renier R Alessandrini Marco M Perny Michael M Roccio Marta M Speck Roberto F RF Senn Pascal P Krause Karl Heinz KH
Molecular therapy. Nucleic acids 20181214
Synthetic microRNA (miRNA) minigenes (SMIGs) have a major potential for molecular therapy; however, their optimal architecture still needs to be determined. We have previously optimized the stem structure of miRNA hairpins for efficient gene knockdown. Here, we investigate the overall architecture of SMIGs driven by polymerase II-dependent promoters. When miRNA hairpins were placed directly behind the promoter, gene knockdown was inefficient as compared with constructs containing an intercalated ...[more]