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Development of a peptide-based fluorescent probe for biological heme monitoring.

ABSTRACT: Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.

SUBMITTER: Newton LD

PROVIDER: S-EPMC6350759 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Development of a peptide-based fluorescent probe for biological heme monitoring.

Newton Laura D LD Pascu Sofia I SI Tyrrell Rex M RM Eggleston Ian M IM

Organic & biomolecular chemistry 20190101 3

Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation. ...[more]

PMID: 30574967

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