Ontology highlight
ABSTRACT:
SUBMITTER: Suckling L
PROVIDER: S-EPMC6352296 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Suckling Lorna L McFarlane Ciaran C Sawyer Chelsea C Chambers Stephen P SP Kitney Richard I RI McClymont David W DW Freemont Paul S PS
Synthetic and systems biotechnology 20190122 1
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use of the Design of Experiments (DOE) methodology to optimise the miniaturised preparation of plasmid DNA libraries for NGS, using the Illumina<sup>®</sup> Nextera XT technology and the Labcyte Echo<sup> ...[more]