Dataset Information

Biotic priming with Pseudomonas fluorescens induce drought stress tolerance in Abelmoschus esculentus (L.) Moench (Okra).

ABSTRACT: Drought is a major abiotic stress which negatively affects plant growth and yield. The decrease in rainfall and ensuing drought affect crop production to a larger extent. The present investigation was aimed to evaluate the drought stress mitigation potential of Abelmoschus esculentus (L.) Moench (okra) plants primed with Pseudomonas fluorescens (PF). Okra seeds were primed with 10^-7 CFU of PF, germinated and the plants were exposed to drought stress for 7 days, and the recovery potential was assessed after re-watering the plants. Physiological and biochemical parameters were evaluated during stress and recovery. PF treated plants mitigated the effect of drought stress by increasing relative water content (RWC), accumulated metabolites such as sugar, free amino acids and enhanced the activity of non enzymatic antioxidants; phenolics, ascorbate (AsA) and glutathione (GSH) and reactive oxygen species scavenging enzyme like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guaicol peroxidase (GPX). Drought stress related membrane damage and protein denaturation were also alleviated by PF treatment. During re-watering, PF treated plants regained RWC, total sugar, total amino acid, protein, AsA, GSH, phenolics, SOD, CAT, APX and GPX to appreciable levels. Thus, this study suggests that PF can be used as an agent to effectively mitigate drought stress in okra plants.

SUBMITTER: Pravisya P

PROVIDER: S-EPMC6352537 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Biotic priming with Pseudomonas fluorescens induce drought stress tolerance in Abelmoschus esculentus (L.) Moench (Okra).

Pravisya P P Jayaram K M KM Yusuf A A

Physiology and molecular biology of plants : an international journal of functional plant biology 20181129 1

Drought is a major abiotic stress which negatively affects plant growth and yield. The decrease in rainfall and ensuing drought affect crop production to a larger extent. The present investigation was aimed to evaluate the drought stress mitigation potential of Abelmoschus esculentus (L.) Moench (okra) plants primed with Pseudomonas fluorescens (PF). Okra seeds were primed with 10-7 CFU of PF, germinated and the plants were exposed to drought stress for 7 days, and the r ...[more]

PMID: 30804633

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Project description:As climate changes increase, drought stress is becoming a problem for all major horticultural crops; among them is okra (Abelmoschus esculentus). Despite its superior resilience to heat stress and high nutritional content, it is still underutilized in contrast to other vegetable crops. Moreover, the drought-resistant and drought-sensitive genotypes of okra are also not well known and require further exploration to improve their productivity. To investigate this in more detail, we performed comparative physiological and large-scale chloroplast proteomics on drought-stressed genotypes of okra. We evaluated four major genotypes of okra, viz., NS7774, NS7772, Green Gold, and OH3312 for drought resilient rootstock. The physiological modulations demonstrated a significant change by 50-76% in biomass, net-photosynthetic machinery, water transport, and absorption both in early and late stages of drought stress compared to well-watered crops in all genotypes. Maximum oxidative damage due to drought stress was observed for the genotypes NS7772, Green Gold and OH3312 as depicted by H2O2 and O2- determination. Greater oxidative stress was correlated to lesser antioxidant activity and expression of antioxidant enzymes, such as catalase and ascorbate peroxidase under stress in okra genotypes. The overall photosynthetic pigments, such as total chlorophyll, and total carotenoid content, were also decreased, and stomatal guard cells were disrupted and appeared closed compared to the control for the above three mentioned genotypes, except NS7774. A subsequent tissue-specific proteome analysis of chloroplasts and thylakoids analyzed by BN-PAGE (blue native polyacrylamide gel electrophoresis) revealed either over or under expression of specific proteins, such as ATPase, PSI, PSII core dimer, PSII monomer and ATP synthase. The expression of multiprotein complex proteins, including PSII-core dimer and PSII-core monomer, was slightly higher for the genotype NS7774 when compared to three other genotypes for both 5 and 10 days of drought stress. Further identification of specific proteins obtained in second dimension BN-PAGE provided descriptive detail of seven proteins involved in drought resistance across all genotypes. The identified proteins are majorly involved in photosynthesis under drought stress, suggesting NS7774 as a drought tolerant genotype. Further, the proteomic results were confirmed using Immunoblot by selecting specific protein such as PsaA. Overall, from our physiological modulations and chloroplast proteomics in all genotypes, we summarized NS7774 as a resilient rootstock and the other three genotypes (NS7772, OH3312, and Green Gold) as sensitive ones.

Dataset Information

Biotic priming with Pseudomonas fluorescens induce drought stress tolerance in Abelmoschus esculentus (L.) Moench (Okra).

Publications

Biotic priming with <i>Pseudomonas fluorescens</i> induce drought stress tolerance in <i>Abelmoschus esculentus</i> (L.) Moench (Okra).

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