Molecular safeguarding of CRISPR gene drive experiments.
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ABSTRACT:
SUBMITTER: Champer J
PROVIDER: S-EPMC6358215 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Publications
Molecular safeguarding of CRISPR gene drive experiments.
Champer Jackson J Chung Joan J Lee Yoo Lim YL Liu Chen C Yang Emily E Wen Zhaoxin Z Clark Andrew G AG Messer Philipp W PW
eLife 20190122
CRISPR-based homing gene drives have sparked both enthusiasm and deep concerns due to their potential for genetically altering entire species. This raises the question about our ability to prevent the unintended spread of such drives from the laboratory into a natural population. Here, we experimentally demonstrate the suitability of synthetic target site drives as well as split drives as flexible safeguarding strategies for gene drive experiments by showing that their performance closely resemb ...[more]
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