Safeguarding CRISPR-Cas9 gene drives in yeast.
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ABSTRACT:
SUBMITTER: DiCarlo JE
PROVIDER: S-EPMC4675690 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
Safeguarding CRISPR-Cas9 gene drives in yeast.
DiCarlo James E JE Chavez Alejandro A Dietz Sven L SL Esvelt Kevin M KM Church George M GM
Nature biotechnology 20151116 12
RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the prospect of unanticipated ecological change, demands caution. We report the efficacy of CRISPR-Cas9 gene drive systems in wild and laboratory strains of the yeast Saccharomyces cerevisi ...[more]
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