Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide.
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ABSTRACT:
SUBMITTER: Hazim RA
PROVIDER: S-EPMC6360117 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
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Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide.
Hazim Roni A RA Volland Stefanie S Yen Alice A Burgess Barry L BL Williams David S DS
Experimental eye research 20181015
Human RPE cell lines, especially the ARPE-19 cell line, are widely-used in eye research, as well as general epithelial cell studies. In comparison with primary RPE cells, they offer relative convenience and consistency, but cultures derived from these lines are typically not well differentiated. We describe a simple, rapid method to establish cultures from ARPE-19 cells, with significantly improved epithelial cell morphology and cytoskeletal organization, and RPE-related functions. We identify t ...[more]
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