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DESI Spray Stability in the Negative Ion Mode Is Dependent on Relative Humidity.

ABSTRACT: Ambient ionization mass spectrometry (MS) techniques, such as desorption electrospray ionization (DESI), have been increasingly used due to their simplicity, minimal sample preparation requirements, and potential applications in the field and the clinic. However, due to their intrinsic nature, the performance of these methods is susceptible to variations in ambient conditions. Here, we present data that suggests DESI-MS analysis becomes inconsistent below a relative humidity (RH) level of ~?35%. At low RH, we hypothesize that the DESI spray is subjected to frequent electrical discharges, resulting in unstable ionization and atypical mass spectra. Consequentially, poor image quality is observed when used for tissue imaging. Our results suggest that RH control should be considered in DESI-MS experiments to assure data quality. Graphical Abstract ?.

SUBMITTER: Feider CL

PROVIDER: S-EPMC6362844 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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DESI Spray Stability in the Negative Ion Mode Is Dependent on Relative Humidity.

Feider Clara L CL DeHoog Rachel J RJ Sans Marta M Zhang Jialing J Krieger Anna A Eberlin Livia S LS

Journal of the American Society for Mass Spectrometry 20181219 2

Ambient ionization mass spectrometry (MS) techniques, such as desorption electrospray ionization (DESI), have been increasingly used due to their simplicity, minimal sample preparation requirements, and potential applications in the field and the clinic. However, due to their intrinsic nature, the performance of these methods is susceptible to variations in ambient conditions. Here, we present data that suggests DESI-MS analysis becomes inconsistent below a relative humidity (RH) level of ~ 35%. ...[more]

PMID: 30569427

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