Intestinal electrical stimulation attenuates hyperglycemia and prevents loss of pancreatic ? cells in type 2 diabetic Goto-Kakizaki rats.

ABSTRACT: BACKGROUND/OBJECTIVE:Recently, intestinal electrical stimulation (IES) has been reported to result in weight loss; however, it is unclear whether it has a therapeutic potential for diabetes. The aim of the present study was to explore the potential hypoglycemic effects of IES and its possible mechanisms involving ? cells in diabetic rats. SUBJECTS/METHODS:Diabetic Goto-Kakizaki (GK) rats were chronically implanted with one pair of electrodes in the duodenum. The oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed with or without IES, and plasma glucagon-like peptide-1 (GLP-1) and insulin level were measured. In the other two OGTT sessions, rats were treated with either Exendin (9-39) (GLP-1 antagonist) or Exendin (9-39) plus IES to investigate the underlying mechanism involving GLP-1. Gastric emptying and small intestinal transit were also measured with or without IES. In a chronic study, GK rats were treated with IES or Sham-IES for 8 weeks. Blood glucose, plasma GLP-1 and insulin level, body weight, and food intake were measured. Pancreas weight, islet ?-cell apoptosis, and proliferation were also analyzed. RESULTS:Acute IES reduced blood glucose level from 60 to 120?min during OGTT by 16-20% (all p?

SUBMITTER: Ouyang X 

PROVIDER: S-EPMC6365494 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature