New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures.
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ABSTRACT:
SUBMITTER: Puente-Munoz V
PROVIDER: S-EPMC6367440 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Publications
New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures.
Puente-Muñoz Virginia V Paredes José M JM Resa Sandra S Vílchez José Damaso JD Zitnan Michal M Miguel Delia D Girón María Dolores MD Cuerva Juan M JM Salto Rafael R Crovetto Luis L
Scientific reports 20190207 1
A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescenc ...[more]
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