A Novel Ruthenium-based Molecular Sensor to Detect Endothelial Nitric Oxide.

ABSTRACT: Nitric oxide (NO) is a key regulator of endothelial cell and vascular function. The direct measurement of NO is challenging due to its short half-life, and as such surrogate measurements are typically used to approximate its relative concentrations. Here we demonstrate that ruthenium-based [Ru(bpy)₂(dabpy)]²⁺ is a potent sensor for NO in its irreversible, NO-bound active form, [Ru(bpy)₂(T-bpy)]²⁺. Using spectrophotometry we established the sensor's ability to detect and measure soluble NO in a concentration-dependent manner in cell-free media. Endothelial cells cultured with acetylcholine or hydrogen peroxide to induce endogenous NO production showed modest increases of 7.3 ± 7.1% and 36.3 ± 25.0% respectively in fluorescence signal from baseline

SUBMITTER: Vidanapathirana AK 

PROVIDER: S-EPMC6368587 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature