Ontology highlight
ABSTRACT:
SUBMITTER: Pomatto MAC
PROVIDER: S-EPMC6370572 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Pomatto Margherita Alba Carlotta MAC Bussolati Benedetta B D'Antico Sergio S Ghiotto Sara S Tetta Ciro C Brizzi Maria Felice MF Camussi Giovanni G
Molecular therapy. Methods & clinical development 20190109
Extracellular vesicles (EVs) carry various molecules involved in intercellular communication and have raised great interest as drug delivery systems. Several engineering methods have been investigated for vesicle loading. Here, we studied the electroporation of EVs isolated from plasma to load antitumor microRNAs (miRNAs). First, we optimized the transfection protocol using miRNA cel-39 by evaluating different parameters (voltage and pulse) for their effect on vesicle morphology, loading capacit ...[more]