Ontology highlight
ABSTRACT:
SUBMITTER: Waner MJ
PROVIDER: S-EPMC6372923 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Waner Mark J MJ Hiznay James M JM Mustovich Anthony T AT Patton William W Ponyik Charles C Mascotti David P DP
Biochemistry and biophysics reports 20190103
While the binding of biotin by streptavidin does not appear to be cooperative in the traditional sense of altered binding strength, it has been suggested that it may be cooperative in terms of differential structural changes in the protein. In this work we present intrinsic tryptophan fluorescence data as evidence of a cooperative structural change. The technique involves examination of the differences in fluorescence emission corresponding to distinct tryptophan populations accompanying protein ...[more]