Ontology highlight
ABSTRACT:
SUBMITTER: Mittal R
PROVIDER: S-EPMC3059817 | biostudies-other | 2011 Mar
REPOSITORIES: biostudies-other
Bioconjugate chemistry 20110211 3
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to their performance in cell tagging, tracking, and imaging experiments. Commercially available streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD-biomolecule conjugates. The fluorescence quenching of biotin-4-fluorscein (B4F) provides a straightforward assay to quantify the number of biotin binding sites per SAv QD. The utility of this method was demonstrated by quantitativel ...[more]