Dataset Information

Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model.

ABSTRACT:

Introduction

Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication.

Methods

An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in Pichia pastoris, a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT-ALT-2 in Balb/c mice.

Result and discussion

P-TUFT-ALT-2 showed an enhanced IgG peak titre compared to E. coli expressed E-ALT-2 and P. pastoris expressed P-ALT-2. IgG2b, IgG2a and IgG1 production were predominant indicating a balanced Th1/Th2 response. P-TUFT-ALT-2 also induced about 28% and 9.5% higher splenocyte proliferation over control and E-ALT-2 respectively. Splenocytes produced predominant IFN-γ followed by IL-5, IL-2 and IL-10 specifying a balanced Th1/Th2 response. P-TUFT-ALT-2 showed 55% to 80% with an average of 65% cytotoxicity in B. malayi L3 larvae in in vitro ADCC assay.

Conclusion

This experiment validates P-TUFT-ALT-2 as a potential vaccine candidate for human lymphatic filariasis.

SUBMITTER: Paul R

PROVIDER: S-EPMC6378782 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Publications

Immunological evaluation of fusion protein of <i>Brugia malayi</i> abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model.

Paul Rajkumar R Ilamaran Meganathan M Khatri Vishal V Amdare Nitin N Reddy Maryada Venkata Rami MVR Kaliraj Perumal P

Parasite epidemiology and control 20190207

<h4>Introduction</h4>Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication.<h4>Methods</h4>An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in <i>Pichia pastoris</i>, a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT- ...[more]

PMID: 30847408

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