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Slow Ca2+ Efflux by Ca2+/H+ Exchange in Cardiac Mitochondria Is Modulated by Ca2+ Re-uptake via MCU, Extra-Mitochondrial pH, and H+ Pumping by FOF1-ATPase.


ABSTRACT: Mitochondrial (m) Ca2+ influx is largely dependent on membrane potential (??m), whereas mCa2+ efflux occurs primarily via Ca2+ ion exchangers. We probed the kinetics of Ca2+/H+ exchange (CHEm) in guinea pig cardiac muscle mitochondria. We tested if net mCa2+ flux is altered during a matrix inward H+ leak that is dependent on matrix H+ pumping by ATPm hydrolysis at complex V (FOF1-ATPase). We measured [Ca2+]m, extra-mitochondrial (e) [Ca2+]e, ??m, pHm, pHe, NADH, respiration, ADP/ATP ratios, and total [ATP]m in the presence or absence of protonophore dinitrophenol (DNP), mitochondrial uniporter (MCU) blocker Ru360, and complex V blocker oligomycin (OMN). We proposed that net slow influx/efflux of Ca2+ after adding DNP and CaCl2 is dependent on whether the ?pHm gradient is/is not maintained by reciprocal outward H+ pumping by complex V. We found that adding CaCl2 enhanced DNP-induced increases in respiration and decreases in ??m while [ATP]m decreased, ?pHm gradient was maintained, and [Ca2+]m continued to increase slowly, indicating net mCa2+ influx via MCU. In contrast, with complex V blocked by OMN, adding DNP and CaCl2 caused larger declines in ??m as well as a slow fall in pHm to near pHe while [Ca2+]m continued to decrease slowly, indicating net mCa2+ efflux in exchange for H+ influx (CHEm) until the ?pHm gradient was abolished. The kinetics of slow mCa2+ efflux with slow H+ influx via CHEm was also observed at pHe 6.9 vs. 7.6 by the slow fall in pHm until ?pHm was abolished; if Ca2+ reuptake via the MCU was also blocked, mCa2+ efflux via CHEm became more evident. Of the two components of the proton electrochemical gradient, our results indicate that CHEm activity is driven largely by the ?pHm chemical gradient with H+ leak, while mCa2+ entry via MCU depends largely on the charge gradient ??m. A fall in ??m with excess mCa2+ loading can occur during cardiac cell stress. Cardiac cell injury due to mCa2+ overload may be reduced by temporarily inhibiting FOF1-ATPase from pumping H+ due to ??m depolarization. This action would prevent additional slow mCa2+ loading via MCU and permit activation of CHEm to mediate efflux of mCa2+. HIGHLIGHTS -We examined how slow mitochondrial (m) Ca2+ efflux via Ca2+/H+ exchange (CHEm) is triggered by matrix acidity after a rapid increase in [Ca2+]m by adding CaCl2 in the presence of dinitrophenol (DNP) to permit H+ influx, and oligomycin (OMN) to block H+ pumping via FOF1-ATP synthase/ase (complex V).-Declines in ??m and pHm after DNP and added CaCl2 were larger when complex V was blocked.-[Ca2+]m slowly increased despite a fall in ??m but maintained pHm when H+ pumping by complex V was permitted.-[Ca2+]m slowly decreased and external [Ca2+]e increased with declines in both ??m and pHm when complex V was blocked.-ATPm hydrolysis supports a falling pHm and redox state and promotes a slow increase in [Ca2+]m.-After rapid Ca2+ influx due to a bolus of CaCl2, slow mCa2+ efflux by CHEm occurs directly if pHe is low.

SUBMITTER: Haumann J 

PROVIDER: S-EPMC6378946 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Slow Ca<sup>2+</sup> Efflux by Ca<sup>2+</sup>/H<sup>+</sup> Exchange in Cardiac Mitochondria Is Modulated by Ca<sup>2+</sup> Re-uptake via MCU, Extra-Mitochondrial pH, and H<sup>+</sup> Pumping by F<sub>O</sub>F<sub>1</sub>-ATPase.

Haumann Johan J   Camara Amadou K S AKS   Gadicherla Ashish K AK   Navarro Christopher D CD   Boelens Age D AD   Blomeyer Christoph A CA   Dash Ranjan K RK   Boswell Michael R MR   Kwok Wai-Meng WM   Stowe David F DF  

Frontiers in physiology 20190204


Mitochondrial (m) Ca<sup>2+</sup> influx is largely dependent on membrane potential (ΔΨ<sub>m</sub>), whereas mCa<sup>2+</sup> efflux occurs primarily via Ca<sup>2+</sup> ion exchangers. We probed the kinetics of Ca<sup>2+</sup>/H<sup>+</sup> exchange (CHE<sub>m</sub>) in guinea pig cardiac muscle mitochondria. We tested if net mCa<sup>2+</sup> flux is altered during a matrix inward H<sup>+</sup> leak that is dependent on matrix H<sup>+</sup> pumping by ATP<sub>m</sub> hydrolysis at complex V (F  ...[more]

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