Ontology highlight
ABSTRACT:
SUBMITTER: Chen SH
PROVIDER: S-EPMC6379666 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Chen Shih-Hsun SH Yu Xiaochun X
Nucleic acids research 20190201 3
All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP) for DNA ligation. Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ligation. In the in vitro ligation assays, we show that the recombinant Ligase IV can use both ATP and NAD+ for DNA ligation. For NAD+-mediated ligation, the BRCA1 C-terminal (BRCT) domain of Ligase IV recognizes NAD+ and facilitates the adenylation ...[more]