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Conformational flexibility in the enterovirus RNA replication platform.

ABSTRACT: A presumed RNA cloverleaf (5'CL), located at the 5'-most end of the noncoding region of the enterovirus genome, is the primary established site for initiation of genomic replication. Stem-loop B (SLB) and stem-loop D (SLD), the two largest stem-loops within the 5'CL, serve as recognition sites for protein interactions that are essential for replication. Here we present the solution structure of rhinovirus serotype 14 5'CL using a combination of nuclear magnetic resonance spectroscopy and small-angle X-ray scattering. In the absence of magnesium, the structure adopts an open, somewhat extended conformation. In the presence of magnesium, the structure compacts, bringing SLB and SLD into close contact, a geometry that creates an extensive accessible major groove surface, and permits interaction between the proteins that target each stem-loop.

SUBMITTER: Warden MS

PROVIDER: S-EPMC6380274 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Conformational flexibility in the enterovirus RNA replication platform.

Warden Meghan S MS Cai Kai K Cornilescu Gabriel G Burke Jordan E JE Ponniah Komala K Butcher Samuel E SE Pascal Steven M SM

RNA (New York, N.Y.) 20181221 3

A presumed RNA cloverleaf (5'CL), located at the 5'-most end of the noncoding region of the enterovirus genome, is the primary established site for initiation of genomic replication. Stem-loop B (SLB) and stem-loop D (SLD), the two largest stem-loops within the 5'CL, serve as recognition sites for protein interactions that are essential for replication. Here we present the solution structure of rhinovirus serotype 14 5'CL using a combination of nuclear magnetic resonance spectroscopy and small-a ...[more]

PMID: 30578285

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