Ontology highlight
ABSTRACT:
SUBMITTER: Dong J
PROVIDER: S-EPMC6381110 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Nature communications 20190219 1
Phosphatidylinositol phosphates (PIPs) and cholesterol are known to regulate the function of late endosomes and lysosomes (LELs), and ORP1L specifically localizes to LELs. Here, we show in vitro that ORP1 is a PI(4,5)P<sub>2</sub>- or PI(3,4)P<sub>2</sub>-dependent cholesterol transporter, but cannot transport any PIPs. In cells, both ORP1L and PI(3,4)P<sub>2</sub> are required for the efficient removal of cholesterol from LELs. Structures of the lipid-binding domain of ORP1 (ORP1-ORD) in comple ...[more]