Ontology highlight
ABSTRACT:
SUBMITTER: Gilbert NC
PROVIDER: S-EPMC6382279 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Methods in enzymology 20180328
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered "pseudo wild-type" enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes. ...[more]