Ontology highlight
ABSTRACT:
SUBMITTER: Yen GS
PROVIDER: S-EPMC6383768 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Yen Gloria S GS Fujimoto Bryant S BS Schneider Thomas T Kreutz Jason E JE Chiu Daniel T DT
Journal of the American Chemical Society 20190115 4
We present a method to determine the concentration of nucleic acids in a sample by partitioning it into droplets with a nonuniform volume distribution. This digital PCR method requires no special equipment for partitioning, unlike other methods that require nearly identical volumes. Droplets are generated by vortexing a sample in an immiscible oil to create an emulsion. PCR is performed, and droplets in the emulsion are imaged. Droplets with one or more copies of a nucleic acid are identified, a ...[more]