Dataset Information

A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset.

ABSTRACT: Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labeled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, by combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.

SUBMITTER: Lin MK

PROVIDER: S-EPMC6384052 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset.

Lin Meng Kuan MK Takahashi Yeonsook Shin YS Huo Bing-Xing BX Hanada Mitsutoshi M Nagashima Jaimi J Hata Junichi J Tolpygo Alexander S AS Ram Keerthi K Lee Brian C BC Miller Michael I MI Rosa Marcello Gp MG Sasaki Erika E Iriki Atsushi A Okano Hideyuki H Mitra Partha P

eLife 20190205

Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (<i>Callithrix jacchus</i>). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin ...[more]

PMID: 30720427

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Dataset Information

A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset.

Publications

A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset.

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