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Anti-Inflammatory Activity of A Polyphenolic Extract from Arabidopsis thaliana in In Vitro and In Vivo Models of Alzheimer's Disease.

ABSTRACT: Alzheimer's disease (AD) is the most common neurodegenerative disorder and the primary form of dementia in the elderly. One of the main features of AD is the increase in amyloid-beta (A?) peptide production and aggregation, leading to oxidative stress, neuroinflammation and neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory and neuroprotective effects and have been proposed as possible therapeutic agents against AD. Here, we investigated the effects of a polyphenolic extract of Arabidopsis thaliana (a plant belonging to the Brassicaceae family) on inflammatory response induced by A?. BV2 murine microglia cells treated with both A?25?35 peptide and extract showed a lower pro-inflammatory (IL-6, IL-1?, TNF-?) and a higher anti-inflammatory (IL-4, IL-10, IL-13) cytokine production compared to cells treated with A? only. The activation of the Nrf2-antioxidant response element signaling pathway in treated cells resulted in the upregulation of heme oxygenase-1 mRNA and in an increase of NAD(P)H:quinone oxidoreductase 1 activity. To establish whether the extract is also effective against A?-induced neurotoxicity in vivo, we evaluated its effect on the impaired climbing ability of AD Drosophila flies expressing human A?1?42. Arabidopsis extract significantly restored the locomotor activity of these flies, thus confirming its neuroprotective effects also in vivo. These results point to a protective effect of the Arabidopsis extract in AD, and prompt its use as a model in studying the impact of complex mixtures derived from plant-based food on neurodegenerative diseases.

SUBMITTER: Mattioli R

PROVIDER: S-EPMC6387160 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Anti-Inflammatory Activity of A Polyphenolic Extract from <i>Arabidopsis thaliana</i> in In Vitro and In Vivo Models of Alzheimer's Disease.

Mattioli Roberto R Francioso Antonio A d'Erme Maria M Trovato Maurizio M Mancini Patrizia P Piacentini Lucia L Casale Assunta Maria AM Wessjohann Ludger L Gazzino Roberta R Costantino Paolo P Mosca Luciana L

International journal of molecular sciences 20190207 3

Alzheimer's disease (AD) is the most common neurodegenerative disorder and the primary form of dementia in the elderly. One of the main features of AD is the increase in amyloid-beta (Aβ) peptide production and aggregation, leading to oxidative stress, neuroinflammation and neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory and neuroprotective effects and have been proposed as possible therapeutic agents against AD. Here, we investigated the effects of a polyp ...[more]

PMID: 30736391

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Dataset Information

Anti-Inflammatory Activity of A Polyphenolic Extract from Arabidopsis thaliana in In Vitro and In Vivo Models of Alzheimer's Disease.

Publications

Anti-Inflammatory Activity of A Polyphenolic Extract from <i>Arabidopsis thaliana</i> in In Vitro and In Vivo Models of Alzheimer's Disease.

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