Fluid dynamics during bleb formation in migrating cells in vivo.
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ABSTRACT:
SUBMITTER: Goudarzi M
PROVIDER: S-EPMC6391022 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Fluid dynamics during bleb formation in migrating cells in vivo.
Goudarzi Mohammad M Boquet-Pujadas Aleix A Olivo-Marin Jean-Christophe JC Raz Erez E
PloS one 20190226 2
Blebs are cellular protrusions observed in migrating cells and in cells undergoing spreading, cytokinesis, and apoptosis. Here we investigate the flow of cytoplasm during bleb formation and the concurrent changes in cell volume using zebrafish primordial germ cells (PGCs) as an in vivo model. We show that bleb inflation occurs concomitantly with cytoplasmic inflow into it and that during this process the total cell volume does not change. We thus show that bleb formation in primordial germ cells ...[more]
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