Ontology highlight
ABSTRACT:
SUBMITTER: Chase J
PROVIDER: S-EPMC6391071 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Chase Jillian J Catalano Andrew A Noble Alex J AJ Eng Edward T ET Olinares Paul Db PD Molloy Kelly K Pakotiprapha Danaya D Samuels Martin M Chait Brian B des Georges Amedee A Jeruzalmi David D
eLife 20181224
Assembly of bacterial ring-shaped hexameric replicative helicases on single-stranded (ss) DNA requires specialized loading factors. However, mechanisms implemented by these factors during opening and closing of the helicase, which enable and restrict access to an internal chamber, are not known. Here, we investigate these mechanisms in the <i>Escherichia coli</i> DnaB helicase•bacteriophage λ helicase loader (λP) complex. We show that five copies of λP bind at DnaB subunit interfaces and reconfi ...[more]