Ontology highlight
ABSTRACT:
SUBMITTER: Mazumder A
PROVIDER: S-EPMC7355006 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Mazumder Abhishek A Lin Miaoxin M Kapanidis Achillefs N AN Ebright Richard H RH
Proceedings of the National Academy of Sciences of the United States of America 20200622 27
The RNA polymerase (RNAP) trigger loop (TL) is a mobile structural element of the RNAP active center that, based on crystal structures, has been proposed to cycle between an "unfolded"/"open" state that allows an NTP substrate to enter the active center and a "folded"/"closed" state that holds the NTP substrate in the active center. Here, by quantifying single-molecule fluorescence resonance energy transfer between a first fluorescent probe in the TL and a second fluorescent probe elsewhere in R ...[more]