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A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer.


ABSTRACT: Background:An amphiphilic cationic copolymer cholesterol-g-poly(amine-co-ester), namely Chol-g-PMSC-PPDL synthesized in a chemoenzymatic route has been utilized as a carrier for p53 gene delivery to check its antitumor efficacy, using human prostate cancer cell line PC-3 (p53 null) as a model. Materials and methods:The transfection efficiency was measured by quantitative PCR and Western blotting assay. The anti-proliferative effect was detected using MTT method, colony formation assay and Live/Dead staining. The anti-migration effect was evaluated through wound healing and Transwell migration assays. Results:The transfection efficiency assay indicated that the carrier-mediated p53 gene transfection could dramatically enhance the intracellular p53 expression level. Through p53 gene delivery, obvious anti-proliferative effect could be detected which was elucidated to be associated with the simultaneous activation of mitochondrial-dependent apoptosis pathway and cell cycle arrest at G1 phase. Meanwhile, the anti-migration effect could be obtained after p53 gene transfection. Conclusion:Chol-g-PMSC-PPDL-mediated p53 gene transfection could potentially be employed as a promising strategy for achieving effective anti-tumor response.

SUBMITTER: Dong M 

PROVIDER: S-EPMC6391147 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated <i>p53</i> gene delivery for achieving antitumor efficacy in prostate cancer.

Dong Mengmeng M   Chen Jiawen J   Zhang Jiayuan J   Liang Xiao X   Yang Jiebing J   Li Dan D   Li Quanshun Q  

International journal of nanomedicine 20190213


<h4>Background</h4>An amphiphilic cationic copolymer cholesterol-g-poly(amine-co-ester), namely Chol-g-PMSC-PPDL synthesized in a chemoenzymatic route has been utilized as a carrier for <i>p53</i> gene delivery to check its antitumor efficacy, using human prostate cancer cell line PC-3 (p53 null) as a model.<h4>Materials and methods</h4>The transfection efficiency was measured by quantitative PCR and Western blotting assay. The anti-proliferative effect was detected using MTT method, colony form  ...[more]

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