Substrate mediated interaction between pairs of keratocytes: Multipole traction force models describe their migratory behavior.

ABSTRACT: A series of traction force microscopy experiments involving pairs of keratocytes migrating on compliant substrates were analyzed. We observed several instances where keratocytes that are about to collide turn before they touch. We term this phenomenon collision avoidance behavior and we propose that the turning is caused by the substrate mediated elastic interactions between the cells. A multipole analysis of the cell traction reveals that the left-right symmetry of the keratocyte traction pattern is broken during collision avoidance events. The analysis further shows that the cell migration direction reorients before the principal traction dipoles as the cells turn. Linear elasticity theory is used to derive the cell-cell interaction energy between pairs of keratocytes. The traction force

SUBMITTER: Palmieri B 

PROVIDER: S-EPMC6396918 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature