Fluorescent Trimeric Hemagglutinins Reveal Multivalent Receptor Binding Properties.

ABSTRACT: Influenza A virus carries hundreds of trimeric hemagglutinin (HA) proteins on its viral envelope that interact with various sialylated glycans on a host cell. This interaction represents a multivalent binding event that is present in all the current receptor binding assays, including those employing viruses or precomplexed HA trimers. To study the nature of such multivalent binding events, we fused a superfolder green fluorescent protein (sfGFP) to the C-terminus of trimeric HA to allow for direct visualization of HA-receptor interactions without the need for additional fluorescent antibodies. The multivalent binding of the HA-sfGFP proteins was studied using glycan arrays and tissue staining. The HA-sfGFP with human-type receptor specificity was able to bind to a glycan array as the free

SUBMITTER: Nemanichvili N 

PROVIDER: S-EPMC6397626 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature