Novel Hg2+-Selective Signaling Probe Based on Resorufin Thionocarbonate and its ?PAD Application.

ABSTRACT: In this study, a novel resorufin thionocarbonate-based Hg2+-selective signaling probe (RT) for microfluidic paper-based analytical device (?PAD) applications is reported. The designed probe, RT, was readily synthesized by the one-step reaction of resorufin with phenyl thionochloroformate. The RT probe displayed a prominent color change from yellow to pink and a marked turn-on fluorescence signaling behavior exclusively toward the Hg2+ ion. The signaling of RT was due to Hg2+-induced hydrolysis of the phenyl thionocarbonate moiety to form the parent resorufin dye, which restored its spectroscopic properties. In addition, RT exhibited the Hg2+-selective signaling behavior without interference by coexisting environmentally relevant metal ions. The detection limit for Hg2+ in simulated wastewater samples was estimated to be 5.8?×?10-8 M. In particular, an RT-equipped ?PAD prepared using a wax printing technique enabled simple and convenient determination of Hg2+ ions in simulated wastewater samples, with a detection limit of 5.9?×?10-6 M.

SUBMITTER: Choi MG 

PROVIDER: S-EPMC6399246 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature