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Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity.

ABSTRACT: As an essential amino acid, cysteine is involved in various biosynthetic and metabolic processes, such as protein synthesis, hormone synthesis, and redox homeostatic maintenance. Inordinate cysteine levels are often associated with serious diseases. Thus, designing and synthesizing a novel fluorescent probe for determining the concentration of cellular cysteine, which could indirectly monitor the prevalence of these diseases, is essential. We developed a florescence probe P-Cy with good sensitivity for cysteine detection in?vivo. P-Cy only exhibited good response toward cysteine but did not show response toward other biothiols, such as homocysteine (Hcy) and glutathione (GSH). In this study, we used P-Cy by successfully imaging cellular endogenous and exogenous cysteine levels. Furthermore, P-Cy was also performed in mice to detect cysteine level, indicating that P-Cy is a powerful tool for cysteine detection in?situ.

SUBMITTER: Duan Z

PROVIDER: S-EPMC6401529 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Fluorescent Imaging for Cysteine Detection In Vivo with High Selectivity.

Duan Zhuwen Z Zhu Youming Y Yang Yuyun Y He Zixu Z Liu Ju J Li Ping P Wang Hui H Tang Bo B

ChemistryOpen 20190305 3

As an essential amino acid, cysteine is involved in various biosynthetic and metabolic processes, such as protein synthesis, hormone synthesis, and redox homeostatic maintenance. Inordinate cysteine levels are often associated with serious diseases. Thus, designing and synthesizing a novel fluorescent probe for determining the concentration of cellular cysteine, which could indirectly monitor the prevalence of these diseases, is essential. We developed a florescence probe P-Cy with good sensitiv ...[more]

PMID: 30886789

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