Ontology highlight
ABSTRACT:
SUBMITTER: Wang L
PROVIDER: S-EPMC9468011 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Wang Liang L Wu Chunling C Peng Wanling W Zhou Ziliang Z Zeng Jianzhi J Li Xuelin X Yang Yini Y Yu Shuguang S Zou Ye Y Huang Mian M Liu Chang C Chen Yefei Y Li Yi Y Ti Panpan P Liu Wenfeng W Gao Yufeng Y Zheng Wei W Zhong Haining H Gao Shangbang S Lu Zhonghua Z Ren Pei-Gen PG Ng Ho Leung HL He Jie J Chen Shoudeng S Xu Min M Li Yulong Y Chu Jun J
Nature communications 20220912 1
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum ΔF/F<sub>0</sub> of 1100% in HEK293T ...[more]