Ontology highlight
ABSTRACT:
SUBMITTER: Ma X
PROVIDER: S-EPMC6402485 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Ma Xin X Shah Santosh S Zhou Mowei M Park Chad K CK Wysocki Vicki H VH Horton Nancy C NC
Biochemistry 20130617 25
SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence and exhibits self-modulation of DNA cleavage activity and sequence specificity. Previous studies have shown that SgrAI forms large oligomers when bound to particular DNA sequences and under the same conditions where SgrAI exhibits accelerated DNA cleavage kinetics. However, the detailed structure and stoichiometry of the SgrAI-DNA complex as well as the basic building block of the oligomers have not bee ...[more]