Ontology highlight
ABSTRACT:
SUBMITTER: Kim BJ
PROVIDER: S-EPMC6413556 | biostudies-literature | 2019 Mar-Apr
REPOSITORIES: biostudies-literature
Kim Bang-Jin BJ Kim Yong-Hee YH Oh Myeong-Geun MG Kim Ki-Jung KJ Jung Sang-Eun SE Jin Ju-Hee JH Kim Sun-Uk SU Min Kwan-Sik KS Ryu Buom-Yong BY
Asian journal of andrology 20190301 2
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. The ...[more]