Ontology highlight
ABSTRACT:
SUBMITTER: Hamra FK
PROVIDER: S-EPMC7336504 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Hamra F Kent FK Richie Christopher T CT Harvey Brandon K BK
Transgenic research 20170612 4
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long ...[more]